摘要
报道了血红蛋白（Ｈｂ）在裸银电极上的电化学氧化还原行为．在＋０．４～－０．２Ｖ（ｖｓ．ＳＣＥ）电位范围内于ｐＨ＝４．５的０．１ｍｏｌ／ＬＮａＡｃ－ＨＡｃ底液中，血红蛋白产生一对灵敏的氧化还原峰．峰电位之差△Ｅ为０．２５Ｖ（扫描速度２０ｍＶ／ｓ）．动力学研究表明：电极反应的电子转移数ｎ为０．９４，表现电子传递速率常数Ｋｓ为０．０３２．连续电位扫描３０ｍｉｎ，峰电流变化分别为０．２μＡ（还原峰）和０．１５μＡ（氧化峰）．两峰与血红蛋白浓度在２×１０￣（－７）～２×１０－（－６）ｍｏｌ／Ｌ和２×１０￣（－６）～１．５×１０￣（－５）ｍｏｌ／Ｌ范围内均呈良好线性关系，已应用于血红蛋白的分析测定．
This paper reports the electrochemical behaviour of hemoglobin at bare silver electrode.In the potential range of 0.4～-0.2 V(vs.SCE),a pair of redox peaks was observedat +0.26 V(oxidation) and +0.01 V(reduction)respectively at the sweep rate of 20 mV/swith an apparent electron-transfer rate constant(K_s)0.032 s-(-1)and electron-transfer number(n) 0.94.The redox reaction showed such a good stability that the currents changed lessthan 0.2 μA under a continued scan for 30 min,Hemoglobin was adsorped on the electrodesurface,but it was a faint adsorption.The electrode surface was not be stained during theredox reaction,In the medium of 0.1 mol/L NaAc-HAc(pH=4.5),both oxided current andreduced current are linear relationship with the concentration of hemoglobin in the ranges of2×10￣(-7)～2×10￣(-6)mol/L and 2×100￣(-5)～1.5×10￣(-5)mol/L.It can be used as a method for de-termining hemoglobin.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1996年第1期33-37,共5页
Chemical Journal of Chinese Universities
基金
国家自然科学基金
关键词
血红蛋白
直接电化学
裸银电极
Hemoglobin, Direct electrocliemistry, Bare silver electrode
