摘要
本文研究了牛血清白蛋白（ＢＳＡ）在裸银电极上的直接电化学行为，在ｐＨ＝７．０的ＫＨ２ＰＯ４－Ｋ２ＨＰＯ４缓冲溶液中，ＢＳＡ在裸银电极上有一对准可逆的氧化还原峰，当扫描速度为２０ｍＶ／ｓ．ＢＳＡ浓度为１．２×１０－７ｍｏｌ／Ｌ时，峰电位分别为＋０．２０Ｖ（氧化峰）和＋０．０５Ｖ（还原峰）ｖｓ·ＳＣＥ．动力学研究表明：电极反应的电子转移数为１．８９（相当于２个电子反应），表现电子传递速率常数为０．０５６ｓ－１．氧化峰和还原峰电流与ＢＳＡ浓度在６．０×１０－８～８．０×１０－７ｍｏｌ／Ｌ范围内有良好的线性关系。
This paper reports the direct electrochemical behavior of bovine serum albumin (BSA) at bare silver electrodes in medium of phosphate buffer (pH=7.0).In potential range of -0.10￣+0.35V(vs SCE),A Pare of redox peaks are observed at +0.05V(reduction) and +0.20V (Oxidation) respectively.The redox reactions between BSA and silver electrodes are quasi-reversible with 2 election transfer and 0.70 coefficient as well as 0.056 s -1 apparent election-transfer rate constant.Moreover,both cathodic peak currents and anodic peak currents are linear relationship with BSA concentration in the range of 6.0×10 -8 ～8.0×10 -7 mol/L.It is possible to be used as a method for determination of BSA quantitatively.
出处
《郑州大学学报（自然科学版）》
CAS
1997年第1期85-88,共4页
Journal of Zhengzhou University (Natural Science)
基金
国家自然科学基金
关键词
血清
白蛋白
直接电化学
银电极
电化学
BSA
direct electrochernisty,bovine serum albumin,bare silver electrode
