摘要
目的：建立测定人尿中多西环素含量的反相高效液相色谱方法。方法：以盐酸土霉素为内标，色谱分离采用Hypersil-ODS分析柱（4.6mm×250mm 10μm），柱温30℃·以N，N-二甲基甲酰胺-0．05mol·L^-1草酸铵缓冲液（40：60，pH7．5）为流动相；流速：1．0mL·min^-1，紫外检测波长：269nm。结果：尿中多西环素测定方法的线性范围为5～310mg·L^-1，方法回收率平均为99．63％；日内精密度RSD小于0．49％、日间精密度RSD小于0．63％，最低检测限为0．03mg·L^-1。多西环素尿样在检测过程中保持稳定。结论：本方法操作简便，灵敏度高，专一性强，可用于多西环素临床药动学研究。
OBJECTIVE To establish a method for determination of doxycycline hydrochloride in human urine by HPLC. METHODS The oxytetracycline was used as the internal standard. A Hypersil ODS analytical column（4. 6 mm× 250 mm, 10μm） ,was used and the column temperature was 30℃. The mobile phase was a mixture of DMF-0. 05 mol·L^-1 ammonium oxalate buffer of pH 7. 5 （40：60） and the flow rate was 1.0 mL·min^-1. The UV detection wavelength was 269 nm. RESULTS The linear range of doxycycline in urine was 5 - 310 mg·L ^-1. The average recoveries of 3 concentrations were all higher than 97%. The RSD of intra day was less than 1.44%, the RSD of inter-day was no more than 2. 23%. CONCLUSION The method is accurate, specific and reproducible. It is an available method for the pharmacokinetic study of doxycycline hydrochloride in human urine.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2006年第4期447-450,共4页
Chinese Journal of Hospital Pharmacy
