摘要
作者观察了缺氧状态下培养的小牛肺动脉内皮细胞（ＰＡＥＣ）的形态、生长曲线和蛋白质含量的变化，动态测定了缺氧时ＰＡＥＣ细胞内游离钙浓度（［Ｃａ（２＋］ｉ）和ｃＡＭＰ含量。结果发现：（１）ＰＡＥＣ能在２％Ｏ＿２，缺氧条件下生长，其光镜形态、生长曲线和细胞蛋白质含量与２１％Ｏ＿－２常氧条件下的相似；（２）向ＰＡＥＣ的无钙细胞悬液内分别通入氮气、３％Ｏ＿２和５％Ｏ＿２１～２０ｍｉｎ，细胞内（［Ｃａ（２＋］ｉ）均明显升高；（３）通入氮气１ｍｉｎ，细胞内ｃＡＭＰ含量明显上升，继续缺氧则ｃＡＭＰ有向基础值接近的趋势。上述结果提示：培养的ＰＡＥＣ对缺氧有较强的耐受力，缺氧可直接影响ＰＡＥＣ的第二信使传递系统。
The effects of hypoxia on cell growth, cAMP and cytosolic free calcium concentrations([Ca(2+)]i)of cultured bovine pulmonary arterial endothelial eells(PAEC)wereevaluated.The results obtained were as follows:(1)There were not much differenees in cellgro wth characteristic and eell pro tein co ntent between hypo xic(2%O2)and normoxic(21%O2)groups.It seems that hypoxia didn’t cause PAEC hypertrophy or hyperplasia,(2)[Ca(2+)]i of PAEC were determined by fluorescent probe Fura-2,and cAMP by RIAassay.Changes of [Ca(2+)]i were noticed in PAEC suspended in calcium-free medium exposedto 0% O2(pure N2),3%O2 and 5 %O2 hypoxic challenges respectively for 1 to 20 minutes。[Ca(2+)]i in PAEC under hypoxia elevated significantly and continuously up to 400～500nmol/L(P<0.00 1).The coneentration of cAMP increased significantly(P < 0.01)and transiently atthe cnd of 1 min hypoxic challenge (0% O2).These results suggested that hypoxia could ex-ert direct influences upon second messenger system in cultured PAEC.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1996年第4期337-340,共4页
Chinese Journal of Pathophysiology
基金
国家自然科学基金
关键词
血缺氧
内皮细胞
钙
腺苷环-磷酸
Anoxemia·Etidotlielium·Caicium·Adenosine cyclic monophosphate
