摘要
研究了家兔尿中氯胺酮的检验方法。用1mol/L氢氧化钠溶液调正检尿液pH为9—10,上GDX-403柱,以氯仿洗脱。选取209m/Z离子碎片为母离子,进行GC-MS/MS分析,通过与氯胺酮标准品的保留时间及二级质谱图比对定性,外标定量。结果显示氯胺酮在0.02—40μg/mL范围内线性关系良好,所得校准曲线的回归方程为:y=3990.31+47045.2x,r2=0.9995。检出限为20ng/mL。平均回收率为89.36%。
The pH in urine sample was adjusted to 9-10 by NaOH solution,and the ketamine in urine of rabbit was extracted by GDX-403,eluted with chloroform,and determined by GC-MS/MS with parent ion of 209 for qualitative analysis and external standard method for quantitative analysis. The linear range of 0.02-4.0μg/mL,correlation coefficients of 0. 9995,recovery of 89.36% and the limits of detection of 20ng/mL for urine were obtained, respectively.
出处
《光谱实验室》
CAS
CSCD
2006年第2期231-233,共3页
Chinese Journal of Spectroscopy Laboratory
基金
辽宁警官高等专科学校科研基金资助项目
