摘要
用荧光光谱法研究了咖啡因和茶碱两种生物碱药物与牛血清白蛋白(BSA)分子间的相互结合反应。测定了咖啡因和茶碱与BSA反应的结合平衡常数分别为1.673×104L.mol-1,6.802×103L.mol-1。证实了两种生物碱药物与牛血清白蛋白的相互结合作用为单一的静态猝灭过程。
The interation of caffeine and theophylline with bovine serum albumins has been studied by fluorescence spectroscopy. The results indicated that enoxacin could bind with BSA strongly at molar ratio 1 ： 1 and the equilibrium constants were Kc = 1. 673 × 10^4 L · mol^-1 and Kt =6. 802 × 103 L · mol^-1, respectively. Good linear Stern-Volmer lines were observed on the fluorescence of BSA quenched by enoxacin of different concentration, indicating that the combination reaction of enoxacin with BSA is a single static quenching process.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2006年第3期505-508,共4页
Spectroscopy and Spectral Analysis
基金
国家自然科学基金(20275022)资助项目
关键词
咖啡因
茶碱
牛血清白蛋白
荧光光谱
Caffeine
Theophylline
Bovine serum albumin
Fluorescence spectroscopy
