摘要
通过比较5种大孔吸附树脂对印楝素A的吸附率和解吸率，成功地筛选出比较理想的树脂。研究结果表明：XAD-1180树脂对印楝素A有较好的吸附和解吸效果。并对其动态吸附、解吸性能进行了考察，发现较佳的吸附条件为：印楝素A质量浓度2．23mg／mL（溶剂为30％甲醇-水溶液，以下同），流速1BV／h，饱和吸附量4．5—5BV；解吸条件为：以50％、60％、70％的甲醇-水溶液梯度洗脱。一次提纯产品的纯度为85．14％，经过二次提纯的纯度可达93．18％。纯化产物经HPLC-MS进一步确认为印楝素A。
Five types of macroporous resin were studied in order to compare their performances in absorbing and desorbing azadirachtin A. XAD-1180 was considered as the optimal macroporous resin for azadirachtin A. The dynamic adsorption and desorption conditions for purification of azadirachtin A were studied. The appropriate adsorption conditions were： concentration of azadirachtin A 2 mg/mL in 30% methanol solution, flow rate 1 BV/h, amount of saturated adsorption toward azadirachtin A 4 - 5 BV. The desorption conditions were： gradient elution with 50%, 60% and 70% methanol solution at flow rates 1 and 0. 5 BV/h. The purity of azadirachtin A purified by XAD-1180 for one time was 85.15%. Its purity was 93.1% when purified twice. The purified product was further confirmed to be azadirachtin A by HPLC-MS.
出处
《林产化学与工业》
EI
CAS
CSCD
2006年第1期53-56,共4页
Chemistry and Industry of Forest Products
基金
南京中医药大学资源昆虫培育与利用重点实验室资助项目(2002-1)
关键词
印楝素A
大孔吸附树脂
动态吸附
azadirachtin A
macroporous resin
dynamic absorbing
