摘要
目的探讨从大肠癌患者外周血检测循环癌细胞p53表达的临床意义。方法用流式细胞术,对术前128例大肠癌患者外周血循环癌细胞p53异常表达进行检测,实验数据用SPSS统计软件处理。结果大肠癌外周血循环癌细胞p53异常表达与患者淋巴结转移显著相关(P<0.01);p53异常表达与患者肿瘤分化程度密切并呈正相关(r=0.8476,P<0.05);p53异常表达与淋巴结转移在左右结肠癌病例中差异具有统计学意义(P<0.05)。结论外周血中可检出大肠肿瘤细胞生物学标志物p53突变表达,这可为大肠癌预测复发及远端微转移提供了一个有效的手段。
Objective To study the clinical significance of detectingp53 gene mutation expression in colorectal cancer cells of peripheral blood. Methods Flow cytometry （FCM） was used to detectp53 gene mutation expression in peripheral blood cancer cells of 128 patients with colorectal cancer. Experimental data were analyzed by SPSS （v. 11.0） software. Results The lymph node metastasis showed the significant difference statistically （ P 〈 0.01 ） between/753 positive and negative expression in the colorectal cancer patients. The mutation p.53 expression associated with existing histological differentiation （r = 0. 8476, P 〈 0.05）. A lymph node metastasis difference was observed between left and right colorectal cancers of mutationp53 positive expression. Conclusion Detecting the mutationp53 expression in cancer cells of peripheral blood might be helpful to the early diagnosis of colorectal cancer.
出处
《中华医学遗传学杂志》
CAS
CSCD
北大核心
2006年第2期186-188,共3页
Chinese Journal of Medical Genetics
基金
江苏省科技厅基金(BS99056)~~
关键词
循环癌细胞
大肠癌
P53基因
转移
circulating cancer cells
colorectal cancer
p53 gene
metastasis
