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棉花下胚轴离体培养不定根发生的组织学研究 被引量:5

HISTOLOGICAL STUDY ON ADVENTITIOUS ROOT FORMATION IN COTTON HYPOCOTYL CULTURE
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摘要 离体培养棉花的下胚轴切段,直接和间接产生不定根。组织学观察表明,由下胚轴直接发生的不定根起源于形成层较外围的薄壁细胞、中鞘细胞和近中柱鞘的薄壁细胞,表现出广泛的发生部位而异于棉花侧根发生;由愈伤组织间接产生的不定根也有多种发生途径。不定根的发生过程可分为根原基发生和根原基发育两个阶段,NAA促进根原基发生,却抑制根原基的发育; On the basis of direct and indirect adventitious root formation obtained in cotton hypocotyl culture and its callus culture, a systematic histological study was made in this paper. Direct adventitious roots originated from the exothecium of the procabium in hypocotyl segments, pericycle cells and other cells in primary vascular tissues, which showed a wide origin in position differing from that of lateral roots. Indirect adventitious root formation still had three ways. The process of adventitious root formation could be divided into two stages. initiation and development of root primordiums. NAA promoted the initiation and supressed its further development, while both initiation and development of root primordiums were supressed by KT.
出处 《莱阳农学院学报》 1996年第2期88-94,共7页 Journal of Laiyang Agricultural College
关键词 棉花 下胚轴培养 不定根 cotton hypocotyl culture adventitious root morphology and anatomy
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  • 1Thomas W. Zimmerman,Carol D. Robacker. Media and gelling agent effect on cotton callus initiation from excised seed hypocotyls[J] 1988,Plant Cell, Tissue and Organ Culture(3):269~274

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