摘要
目的了解人精子冷冻前后超微结构及受精能力的变化。方法对10名志愿者的精液分别在冷冻前、冷冻后进行人精子低渗膨胀试验(HOS)、活动率及穿卵率(SPA)测定,并利用电镜对精子进行微细结构观察。结果冷冻后SPA及精子活动率与冷冻前相比显著下降(P<0.05),精子微细结构的变化主要局限于头部顶体,而精子形态、生物膜及亚细胞结构基本完整。结论人精子冷冻后虽然微细结构及功能受到一定程度的损伤,但部分精子的顶体内膜和中纬段未丢失,精子尾部的动力装置未受到损害。
Objective To explore the alteration of ultrastrueture and fertility of human sperm before and after frozen. Methods Sperm from 10 volunteers were evaluated for abilities of respond to sperm hypoosmotie tail swelling test （HOS） and sperm penetration assay （SPA） before and after frozen respectively. Then the ultrastrueture of sperm was observed using electron microscope. Results Abilities to HOS, SPA and motility of frozen sperm were significantly lower than those before frozen （p 〈 0.05）. Ultrastructure alteration was limited to acrosome, but sperm morphology, biomembrane and subcellular structure were normal completely. Conclusion Although ultrastrueture and ability of frozen sperm are damaged, spermatozoa have fertility if inner aerosomal membrane and the equatorial segment are not lost and the dynamic device of sperm tail is not damaged.
出处
《浙江预防医学》
2006年第3期4-5,共2页
Zhejiang Journal of Preventive Medicine
关键词
冷冻精子
受精能力
人精子低渗膨胀试验
超微结构
穿卵试验
Frozen sperm
Fertility
Sperm hypoosmotic tail swelling test
Sperm penetration assay
Uhrastructure
