摘要
目的检测FasFasL在各阶段婴幼儿血管瘤组织中的表达,探讨FasFasL在婴幼儿血管瘤细胞凋亡中的作用。方法应用EnVision法免疫组化染色和RT-PCR检测FasFasL蛋白及mRNA在各阶段血管瘤组织中的表达。结果①增生早期和增生中期,部分血管瘤细胞表达Fas;增生晚期,阳性细胞明显增多,FasmRNA表达最强;消退早期,仍有少量微血管内皮细胞表达Fas,之后Fas表达迅速减弱。②最早期细胞团中没有FasL(+)细胞;增生中期,血管瘤组织中出现少量FasL(+)细胞;增生晚期FasL(+)细胞显著增多,FasLmRNA表达最强;消退早期之后,FasL(+)细胞迅速减少以至消失。结论FasFasL与婴幼儿血管瘤演变过程有密切联系,FasFasL介导的血管瘤细胞凋亡可能是婴幼儿血管瘤自行消退的重要原因。
Objective To investigate the expression of Fas/FasL in infantile hemangiomas and discuss the role of Fas/FasL in the pathologic evolution of infantile hemangioma. Method The EnVision immunohistochemical stain and RT-PCR technique was used to examine the expression of Fas/FasL protein and mRNA in the infantile hemangiomas. Results （ 1 ） In the early and middle proliferating stage, a number of infantile hemangioma cells expressed Fas. In the late proliferating stage, the number of positive cells increased obviously and the expression of Fas mRNA was reaching the strongest level. In the early regressing stage the Fas still existed in some cells and after that the expression decreased quickly. （2） Up to the middle proliferating stage, there were a few of FasL（ ＋ ） cells foound. In the late proliferating stage, the number of FasL（ ＋ ） cells increased significantly. From the early regressing stage, the number of FasL（ ＋ ） cells decreased rapidly and disappeared. Conclusion There may exist significant correlation between the expression of Fas/FasL and the development of the infantile hemangioma cells. The apoptosis of the infantile hemangilma cells mediated by Fas/ FasL may be the major reason of the spontaneous involution of infantile bemangioma.
出处
《中华整形外科杂志》
CAS
CSCD
北大核心
2006年第2期116-120,共5页
Chinese Journal of Plastic Surgery
