摘要
目的:探讨脊柱侧凸患者顶椎区椎间盘纤维环中多聚蛋白多糖的表达及其意义。方法:采集2003年7月至2004年9月间行前路松解或矫形手术的40例脊柱侧凸患者侧凸顶椎区椎间盘组织。青少年特发性脊柱侧凸患者(adolescentidiopathicscoliosis,AIS)25例,胸椎椎间盘11例,腰椎椎间盘14例;先天性脊柱侧凸患者(congenitalscoliosis,CS)15例,胸椎椎间盘6例,腰椎椎间盘9例。利用RT-PCR扩增多聚蛋白多糖(Aggre-can),琼脂糖凝胶电泳,在UVP(紫外光测定法)成像系统进行扫描,GelWork图像分析系统中进行灰度测定半定量分析。分别计算AIS组和CS组凹侧、凸侧纤维环中Aggrecan含量,并对CS组和AIS组胸椎和腰椎以及椎间盘的凹侧和凸侧进行比较。结果:AIS组和CS组椎间盘凹侧纤维环中Aggrecan含量低于凸侧,差异有显著性(P<0.01),胸椎椎间盘纤维环中Aggrecan的含量低于腰椎,但无统计学差异。AIS组Aggrecan的含量和CS组相应部位Aggrecan的含量无明显差别。结论:AIS椎间盘纤维环凹凸侧存在Aggrecan代谢差异,并且可能是脊柱侧凸所致的继发改变,但也可能是脊柱侧凸发生、发展中的重要因素。
Objective：To study the expression of the Aggrecan in the concave and convex side of intervertebral disc（apex of the curve） for scoliosis.Method：40 cases of scoliosis including 25 cases of adolescent idiopathic scoliosis（AIS,11 thoracic and 14 lumbar intervertebral disc） and 15 cases of congenital scoliosis（CS,6 thoracic and 9 lumbar intervertebral disc ）.Specimens of the intervertebral disc（apex of the curve） were harvested during anterior surgery and total RNA were extracted.The amplification reaction products of Aggrecan were resolved on 1.0% agarose and analyzed through reverse transcription-polymerase chain reaction （RT- PCR）.The gray scale was measured by the UVP imaging system and GelWork image analysis system,the Aggrecan content in concave and convex side of intervertebral disc were compared with One-Way ANOVA and the results were analyzed using Paired Samples T-Test.Result：The content of Aggrecan were significantly decreased in annulus fibrous of concave side compared with that in convex side both in AIS and CS（P〈0.01）, and there was even lower expression in the thoracic intervertebral disc than that in lumbar,which had no statistical difference.There were no difference incontet of Aggrecan in the similar region between AIS and CS. Concluslon：The different Aggrecan metabolism between concave side and convex side in annulus fibrosus may be one of the important correlative factors during the progression of AIS.
出处
《中国脊柱脊髓杂志》
CAS
CSCD
2006年第3期225-228,共4页
Chinese Journal of Spine and Spinal Cord
基金
江苏省135医学重点人才项目(RC2001001)
