摘要
研究了植物激活蛋白对烟草花叶病毒RNA及外壳蛋白的抑制作用。结果表明，烟草经植物激活蛋白处理后，植株体内烟草花叶病毒RNA含量比对照降低28％；用ELISA和Western方法检测烟草花叶病毒外壳蛋白的含量，处理分别比对照减少20．7％和25．0％。植物激活蛋白显著干扰烟草花叶病毒外壳蛋白的体外聚合过程，22—37％条件下其干扰作用达显著水平。
The inhibition of plant activator protein on RNA and coat protein of tobacco mosaic virus （TMV） was studied. The concentration of TMV RNA in the treated tobacco was reduced by 28%. Using ELISA and Western blotting assays, the concentration of TMV coat protein in the treated tobacco was reduced by 20.7% and 25.0% respectively. It was also revealed that the polymerization of TMV coat protein could be interfered distinctly by plant activator protein at 22 - 37 ℃ in vitro.
出处
《中国生物防治》
CSCD
北大核心
2006年第1期63-66,共4页
Chinese Journal of Biological Control
基金
国家863计划(2003AAAA241130)
国家973计划(2003CB11420)
