摘要
目的通过对Beagle犬精液冷冻技术的研究,比较颗粒法以及冻管法(0．5 ml)对冷冻精子复苏率的影响,并比较几种冷冻稀释保护液对犬精液冷冻的影响。方法 5只1岁左右可正常采精的雄性Beagle犬,手握法采集精液,以不同稀释冷冻保护液处理后,分别采用颗粒及冻管法冷冻,检测冻精前的精液量、精子密度、畸形率、解冻后活率、复苏率和顶体完整率。结果采用颗粒法和冻管法的平均冻后活率相应为0．38和0．41,复苏率51．9％和53．9％,顶体完整率36．4％和37．6％, 采用冻管法的效果稍优于颗粒法,但差异不显著。应用冷冻保护剂Ⅲ,冻后活率、复苏率和顶体完整率均优于采用稀释冷冻保护剂Ⅰ和Ⅱ,但只有顶体完整率(40．7％和41．3％)与其它两种保护剂相比达到差异显著的水平。结论采用文献中报道的稀释保护液配方均可获得较好的稀释保护效果。
Objective To assess the effect of frozen methods and cryoprotections on quality of frozen sperm from Beagle dog. Methods Five male Beagle dogs were chosen for collection of semen and 25 fractions of semen samples were diluted with three kinds of extenders and frozen separately using two methods. Result Average post thaw progressive motility were 51.9% and 53.9, and the percentage of sperms with intact acrosome were 36.4% and 37.6%, respectively. The percentage of sperms with intact acrosome supplemented with protect preservative agent III were 40.7 and 41.3 by the frozenstraw method and pellet method, which better than the other agent, but only the percentage of sperms with intact acrosome was significant （P〈0.05）. Conclusion The frozen-straw method was better than the pellet method, but there was no statistical difference. The extender supplemented with vitamin E, B6 and glutamic acid is superior to the other two extenders in the average post thaw progressive motility, the percentages of recovery sperms and the percentage of sperms with intact acrosome, but only the percentage of sperms with intact acrosome was significant （P〈0.05）.
出处
《实验动物与比较医学》
CAS
2006年第1期34-37,共4页
Laboratory Animal and Comparative Medicine
基金
上海农林职业技术学院校内基金项目
