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公鸭、公鸡精子对在液氮中低温冷冻保存耐受力的比较研究 被引量:3

A Comparative Study on Durability in Liquid Nitrogen Cryopreservation between Drake and Cock Spermatozoa
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摘要 比较公鸭和公鸡精子经低温冷冻保存后存活率、顶体完整率方面的差异。结果表明,公鸭冷冻精液解冻后的精子存活率和顶体完整率都不同程度地高于相同类型的公鸡冷冻精液,但差异均未达到显著水准(P>0.05)。 In this paper the differences in survival rate, acrosomic integrity rate of spermatozoa between drake and cock after cryopreservation in liquid nitrogen are compared. The results show that the survival rate and acrosomic integrity rate of drake spermatozoa in straws and pellets are higher than those of the same types of cock frozen semen, but the differences are not significant (n = 20, P 〉 0.05).
出处 《武汉科技大学学报》 CAS 2006年第2期186-188,共3页 Journal of Wuhan University of Science and Technology
基金 国家"863"资助项目(2001AA213081) 湖北省教育厅青年基金课题资助项目(2004D016)
关键词 公鸭精子 公鸡精子 冷冻保存 存活率 顶体完整率 drake spermatozoa cock spermatozoa cryoprservation survival rate acrosomic integrity rate
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参考文献11

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二级参考文献19

  • 1刘建忠,朱艳君,张一玲.洪山公鸡精液冷冻保存方法及效果的研究[J].武汉科技大学学报,2005,28(2):205-207. 被引量:6
  • 2Sexton T J, Fewlass T A. Effects of the diluent components on the fertilizing eatmcity of chicken semen stored at 5 ℃. Poult Sci,1978, 57(1): 277-284.
  • 3王元兴 郎介金.动物繁殖学(第2版)[M].南京:江苏科学技术出版社,1997..
  • 4Tselutin K, Seigneurin F, Blesbois E. Comparison of cryoproteetants and methods of eryopreservation of fowl spermatozoa.Poult Sci, 1999, 78 (4): 586-590.
  • 5Long J A, Kulkomi G. An effective method for improving the fertility of glycerol-exposed poultry semen. Poult Sci, 2004, 83(9): 1594-1601.
  • 6Chalah T, Brillard J P. Comparison of assessment of fowl sperm viability by Eosin-Nigrosin and dual fluorescence ( SYBR-14/PI). Theriogenology, 1998, 50 (3): 487-493.
  • 7Chalah T, Seigheurin F, Blesbois E, et al. In vitro comparison of fowl sperm viability in ejaculates frozen by three different techniques and relationship with subsequent fertility in vivo.Cryobiology, 1999, 39:185-191.
  • 8Curry M R. Cryopreservation of Semen from Domestic Livestock [J]. Methods Mol Biol, 1995, 38: 189-197.
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  • 10Van Voorst A, Leenstra F R. Fertility Rate of Daily Collected and Cryopreserved Fowl Semen [ J ]. Poult Sci,1995, 74(1): 136-140.

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