摘要
目的研究不同剂量的锰（Mn）对PC12细胞生长增殖的影响。方法体外培养大鼠嗜铬细胞瘤PC12细胞。以100，900μmol／LMnCl2染毒后进行四唑盐比色实验（MTT）观察MnCl2对细胞的抑制作用，TUNEL法观察细胞凋亡，Western blot检测α-共核蛋白（α-SYN）表达情况。结果MTT结果显示100—900μmol／L MnCl2对细胞的生长增殖有显著的抑制作用（P〈0．01）。TUNEL染色结果显示MnCl2处理组TUNEL阳性细胞增多（P〈0．05）。Westem blot结果显示与对照组相比，100、300、500μmol／L MnCl2组α-SYN表达水平分别升高1．8、4．5、7．3倍（P〈0．05）。结论一定剂量的MnCl2对PCl2细胞的生长增殖有明显的抑制作用，这一作用可能是通过诱导胞内α-SYN表达增高实现的。提示预防、阻止α-SYN在细胞中的聚集可能会降低Mn对多巴胺能神经细胞的损伤。
Objective To study the toxicity of different doses of manganese chloride （MnCl2） on rat pheochromocytoma PC12 cells in vitro.Methods PC12 cell cultures were exposed to MnC12 within a dose range of 100-900 μmol/L. The inhibition of the cells during 7 days was determined by MTT assay, the apoptotic cells were monitored by TUNEL stain. The alpha-synuclein were determined by western blot. Results MnCl2 at different concentrations （100, 300, 500, 700, 900 μmol/L） could inhibit the growth and proliferation of PC12 cells in a dose and time-dependent manner and the cell growth curve was changed accordingly. After the treatment of 300,500 μmol/L MnCl2 ,TUNEL-positive cells were also increased （P 〈 0.05）. Westernblot showed that MnCl2 increased the alphasynuclein in a dose-dependent manner （ P 〈 0.05）. Conclusion MnCl2 can significantly inhibit the growth and proliferation of PC12 cells, possibly by inducing apeptosis,and the increase of alpha-synuclein.
出处
《毒理学杂志》
CAS
CSCD
北大核心
2006年第1期4-6,共3页
Journal of Toxicology
基金
国家自然科学基金资助项目(30471434)
