摘要
目的观察硒、锌单独和联合作用对人食管癌细胞株Eca109生长增殖的影响。方法用不同浓度硒、锌培养液培养人食管癌细胞株Eca109,以人正常肝上皮细胞株HL7702为正常细胞对照,采用MTT、3H-TDR掺入实验及流式细胞术研究不同浓度硒、锌对两株细胞生长增殖的影响。结果高浓度硒(0.3μg/ml)、锌(3.5μg/ml)抑制Eca109细胞增殖,且二者联合作用比单独作用强(P<0.05);高浓度硒、锌单独促进HL7702细胞生长,联合则抑制其生长。生理浓度硒、锌(分别为0.1、1.0μg/ml)对该癌细胞株和正常细胞株生长增殖作用与对照相近(P>0.05)。0.3μg/ml硒引起癌细胞S期阻滞,作用不明显(P>0.05),而3.5μg/ml锌引起G1期癌细胞显著增加(P<0.05),二者联合使其出现G1期阻滞,并都促进细胞凋亡,联合作用强于单独作用(P<0.05)。结论高浓度硒、锌抑制人食管癌细胞株Eca109生长增殖,且二者联合作用比单独作用强;该联合作用对人正常肝上皮细胞株可能有一定毒性作用。细胞生长周期改变、促进细胞凋亡可能是硒、锌抑制食管癌细胞生长增殖的机制之一。
Objective To observe the effect of selenium and zinc alone or in combination on the growth and proliferation of human esophageal cancer Eca109 cell line in vitro. Methods Different doses of sodium selenite and zinc sulfate were added into the culture medium of Eca109 cells and normal liver epithelial HL7702 cells （control）, and the changes in the cell growth were assessed by means of cell growth curve, 3H-thymidine incorporation assay and flow cytometry. Results High-concentration selenium （0.3 μg/ml） and zinc （3.5 μg/ml） alone both obviously inhibited Eca109 cell proliferation, and the inhibitory effect was enhanced by a combined treatment. At high concentrations, both selenium and zinc promoted HL7702 cell proliferation, but when combined, they produced inhibitory effect on the cell growth. Selenium and zinc at the physiological concentrations （ 0.1 μg/ml and 1.0 μg/ml, respectively） produced similar effects on Ecal09 cells and the control cells. Selenium at 0.3 μg/ml caused Eca109 cell growth arrest in S phase, but this effect was not statistically significant; 3.5 μg/ml zinc significantly increased the number of Eca109 cells in G1 phase. When combined, 0.3 μg/ml selenium and 3.5 μg/ml zinc caused significant G1 arrest and promoted apoptosis of the cancer cells, an effect stronger than that of either of the agents used alone. Conclusion High-concentration selenium and zinc show a synergetic effect in inducing growth inhibition of human esophageal cancer Eca109 cell line possibly by causing cell cycle arrest and promoting cell apoptosis, and their combined use can be toxic to normal human liver epithelial cells.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2008年第12期2117-2120,共4页
Journal of Southern Medical University
基金
国家自然科学基金(30571559)
关键词
食管癌
硒
锌
细胞增殖
esophageal cancer
selenium
zinc
cell proIiferation
