摘要
目的探讨脐带血来源的树突细胞(CBDC)在体外对LAK(lymphokine activated killer)细胞增殖活性和杀伤活性的影响。方法采用GM-CSF和IL-4联合刺激诱导脐带血单个核细胞分化为CBDC,再将CBDC与同源LAK细胞混合培养,用MTT法测定不同细胞密度的CBDC刺激LAK细胞增殖和杀伤的能力。结果体外诱导出形态典型的CBDC,其具有明显刺激LAK细胞增殖的能力。LAK细胞的增殖和杀伤活性在2种细胞混合比为1∶10时最强。结论CBDC能增强LAK的增殖活性和杀伤活性。
Objective To identify the changes in proliferative activity and cytotoxicity of lymphokine activated killer （LAK） ceils after in vitro co-culture with dendritic ceils from umbilical cord blood（CBDC）. Methods CBDCs were induced by using GM-CSF and IL-4, and then co-cultrued with allogenic LAK at different proportions. Proliferative activity and cytotoxicity of LAK ceils were measured by MTT assay. Results The typical CBDCs were induced in vitro, and the cultured CBDCs stimulated the proliferation and cytotoxicity of LAK cells. The optimal proportion of CBDC and LAK was 1：10, at which the activated LAK showed the highest cytotoxic effect on the tumor ceils. Conclusion The CBDCs can enhance the proliferation and cytotoxicity of LAK ceils.
出处
《基础医学与临床》
CSCD
北大核心
2006年第2期174-176,共3页
Basic and Clinical Medicine
