摘要
壳聚糖是一种富含氨基和羟基的天然高分子聚合物,易成膜,膜的机械性能、透光率好,且膜的表面平整光滑,对叶绿素有较高的富集能力,平衡态时叶绿素在壳聚糖膜和水相两相间的分配系数为9090,重现性好,可以研制成固相微萃取薄膜用于叶绿素的分析。磁力搅拌萃取叶绿素,壳聚糖膜可在80min内达到平衡,用5%NaOH超声洗脱30min内可以洗脱干净。红外光谱和X射线衍射谱图表明萃取叶绿素之后的壳聚糖分子中分子内或分子间的氢键作用力大大减弱,可能是壳聚糖分子中的大量氨基和羟基在萃取叶绿素的过程中起关键作用。
Chitosan is a kind of natural polymers containing plenty of amido and hydoxy. The chitosan membrane is tough, and since the partition coefficient of chlorophyll between chitesan membrance and water is as high as 9 090, the chitosan membrane may be easily manufactured as solid-phase microextraction membrane for the analysis of chlorophyll. The system may reach a complete equilibrium in 80 min. Then the membrane can be desorbed completely in 30 min in 5 % NaOH with ultrasonic. Both IR and XRD indicated that hydrogen bonds between the molecules of chitosan were weakened and amido and hydoxy were considered as the keys during the process of extracting chlorophyll.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2006年第1期11-14,共4页
Spectroscopy and Spectral Analysis
基金
国家高技术研究发展计划("863"计划)(2002AA639390)资助项目
关键词
壳聚糖膜
叶绿素
涂层
Chitosan membrane
Chlorophyll
Coating
