摘要
目的探讨^(99m)Tc-转铁蛋白(Tf)对肝细胞癌的诊断价值。方法以^(99m)Tc标记Tf进行Balb/c小鼠体内生物分布,对Tf受体阻断前后的Balb/c人肝癌细胞系SMMC-7721nu/nu裸鼠进行显像研究,测定SMMC-7721细胞膜Tf受体数及125I-Tf与Tf受体的解离常数(Kd)。结果^(99m)Tc-Tf主要分布于肝、血及肾脏。荷人肝癌SMMC-7721裸鼠注入^(99m)Tc-Tf后18h,肿瘤/血、肿瘤/肝及肿瘤/肌肉的比值分别为24.2、1.7和35.5,非标记Tf抑制前后小鼠肿瘤摄取率的比值为16.3。人肝癌SMMC-7721细胞膜Tf受体数为2.4×105/细胞,Kd为4.46nmol/L。结论^(99m)Tc-Tf在高表达Tf受体的肿瘤病变部位特异性聚积,^(99m)Tc-Tf有望用于肝细胞癌Tf受体显像。
Objective To investigate the feasibility of ^99mTc-transferrin （^99mTc-Tf） as an transferrin receptor imaging agent in nude mice bearing human hepatoma SMMC-7721 tumor. Methods Biodistribution of ^99mTc-Tf in Balb/c mice was assayed, and transferrin receptor imaging in Balb/c nu/nu nude mice bearing human hepatoceUular carcinoma SMMC-7721 xenografts was carried out, with or without inhibition of transferrin receptor with unlabeled transferrin. The receptor radioligand binding assay with ^125I-transferrin as ligand was established, and the number of receptors per cell and the Kd were worked out. Results ^99mTc-Tf was mainly distributed in the liver, blood and kidneys. At 18 h post injection, the ratios of tumor/blood, tumor/liver and tumor/muscle were 24.2, 1.7 and 35.5, respectively. The ratio of tumor uptake at 18 h without unlabeled transferrin pretreatment to that with pretreatment was 16.3. The number of receptors per cellwas 2.4 ×10^5 and the Kd was found to be 4. 4 6 nmol / L. Conclusion All those results indicate that ^99mTc-Tf is specifically localized in tumor lesions of nude mice. ^99mTc-Tf may be useful in detection of hepatocellular carcinoma.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2006年第2期96-98,共3页
Chinese Journal of Oncology
