摘要
目的研究抗CD20嵌合抗体片段Fab′突变体诱导B淋巴细胞凋亡的发生、凋亡相关基因表达以及细胞内钙离子的浓度变化。方法以人B淋巴细胞(Raji细胞)为凋亡细胞模型,通过MTT法、Western blot和流式细胞仪等方法观察bcl-2/bax基因表达、细胞色素c及胞内Ca2+的变化。结果抗CD20嵌合抗体片段Fab′突变体对Raji细胞生长增殖具有明显的抑制作用,并呈剂量依赖性。细胞中bcl-2表达有微弱的下降,而bax表达明显升高,并出现细胞色素c的释放和胞内Ca2+浓度的升高。结论抗CD20嵌合抗体片段Fab′突变体诱导Raji细胞凋亡,与bcl-2/bax表达、细胞色素c的释放和胞内Ca2+浓度的升高有关。
Objective：To investigate the effect of chimeric anti-CD20 antibody Fab＇fragment on apoptosis, apoptosis related genes expression, and intracellular Ca^2＋ concentration of B lymphoma cells. Methods.. The proliferation of human B lymphoma cell line Raji was measured by MTT assay. The release of cytochrome c and expression of bcl-2/bax was detected by Western blotting. Apoptosis of Raji cells was determined by FACS analysis. Intracellular calcium concentration was measured by fluorescence method. Results：Chimeric anti-CD20 antibody Fab＇ fragment inhibited proliferation of Raji cells in a dose-dependent manner, slightly down-regulated bcl-2 protein expression, but significantly up-regulated bax expression. Cytochrome c was released and intracellular Ca^2＋ concentration was increased. Conclusion：Chimeric anti-CD20 antibody Fab＇ fragment induced apoptosis of Raji cells which was related with changes of bcl-2/bax, release of cytochrome c, and increase in intracellular Ca^2＋ concentration.
出处
《肿瘤》
CAS
CSCD
北大核心
2006年第2期116-119,共4页
Tumor
基金
国家自然科学基金资助项目(编号:30400558)
天津市科技攻关计划(编号:05YFGPGX069007)
