摘要
目的:探讨甲状腺激素对大鼠骨骼肌组织(SM)、肝组织(LV)和白色脂肪组织(WAT)中解偶联蛋白-2(UCP2)基因表达的影响。方法:正常雄性Wistar大鼠30只,随机分为对照组、甲状腺功能亢进(甲亢)组和甲状腺功能低下(甲低)组,应用左旋甲状腺素钠和甲巯咪唑造成大鼠甲亢和甲低状态;用放免法检测血清总T3、T4的浓度;用半定量逆转录聚合酶链反应(RT-PCR)检测SM、LV和WAT中UCP2mRNA的表达水平。结果:与对照组相比,甲亢组大鼠SM、LV和WAT中UCP2mRNA的水平分别增加了65%、50%和40%,甲低组大鼠SM、LV和WAT中UCP2mRNA的水平分别降低了9%、24%和20%,差异均具有统计学意义,P<0.05。结论:过量甲状腺激素对SM、LV和WAT中UCP2基因的表达具有上调作用,甲状腺激素水平过低则下调SM、LV和WAT中UCP2基因的表达。
Objective： To investigate the effect of thyroid hormone on uncoupling protein-2 （UCP2） gene expression in skeleton muscle （SM）, liver （LV） and white adipose tissue （WAT） in rats. Methods： Thirty normal male Wistar rats were averaged into three groups at random： control, hyperthyroid and hypothyroid. The hyperthyroid or hypothyroid status was made by administration of L-Thyroxine or methimazol. The serum total T3 and T4 concentrations were measured by radioimmunoassay （RIA）. The RNA levels of UCP2 in SM, LV and WAT were detected by semi-quantitative reverse transcriptase polymerase chain reaction （RT-PCR）. Results： Compared with the normal control, UCP2 mRNA in SM, LV and WAT increased by 65%, 50%, 40% and decreased by 9%, 24%, 20% in hyperthyroid and hypothyroid groups. The differences had statistical significance （P〈0.05）. Conclusion： Gene expression of UCP2 in SM, LV and WAT in rats is up-regulated by excessive thyroid hormone, and is down-regulated by low level of thyroid hormone.
出处
《山东大学学报（医学版）》
CAS
北大核心
2006年第2期196-199,共4页
Journal of Shandong University:Health Sciences
关键词
甲状腺激素类
解偶联蛋白=2
基因表达
逆转录聚合酶链反应
Thyroid hormones
Uncoupling protein-2
Gene expression
Reverse transcriptase polymerase chain reaction
