摘要
通过中棉所 12 与 8891 的杂交及多代自交,获得由 180 个家系构成的重组自交系 F8、F9群体。重组自交系群体、两亲本及 F1于 2002、2003 两年种植; 对株型性状进行了研究,两年共调查了 10 个株型形状。利用该重组自交系群体,采用 SSR 为主体的分子标记构建了遗传连锁图,并对株型性状进行了单位点和双位点水平的 QTL 定位。结果表明,QTL 加性效应和上位性互作效应作为棉花重组自交系株型性状的遗传基础起着重要作用; 中棉所 12 与 8891 间多态性位点偏少,而表型差异较大且其杂交种湘杂棉二号有很强的杂种优势,QTL 互作可部分解释这一现象;结合对产量品质性状的研究结果,认为上位性可能是湘杂棉二号杂种优势的重要遗传基础。
Xiangzamian 2 （XZM2） is the most widely cultivated cotton hybrid in China. By crossing two parents Zhongmiansuo12 and 8891 and upon subsequent selfings, we got F8 and F9 populations having 180 recombinant inbred lines. Ten plant architecture traits were investigated in two years with this population. A genetic map was constructed mainly with SSR markers. Quantitative trait loci （QTL） conditioning plant architecture traits were determined at the single-locus and double-locus levels. The results showed that epistastic effects as well as additive effects of QTL played an important role as the genetic basis of cotton plant architecture. The QTL detected in our research might provide new information on improving plant architecture traits. The polymorphism of molecular markers between ZMS12 and 8891 were quite limited, while significant differences between their phenotypes were found and the hybrid XZM2 expressed high heterosis in yield. All these could be partly explained by the effect of epistatic QTL.
基金
This work was supported by the National Science Foundation for Distinguished Young Scholars (No. 30025029), Chinese National Programs for High Technology Research and Development (863 Program) (No. 2002AA207006) and the Teaching and Research Award Program for Outstanding Young Teachers in Higher Education Institutions Program for Changjiang Scholars and Innovative Research Team in University of MOE, China
