摘要
介绍一种RNA提取方法,该方法以SDS、氯仿和Tris苯酚为主要提取试剂,以LiC l和乙醇为RNA沉淀试剂。分别以柽柳(木本植物)、星星草(草本植物)、天牛(昆虫)、酿酒酵母和白腐菌(真菌)为RNA提取材料,用该方法成功地提取出了它们的总RNA。获得的RNA条带清晰,A260/A280在1.8以上。通过对LiC l和乙醇沉淀RNA的效果分析表明,该方法可在10 m in内完全沉淀RNA,同时也可以同时获得纯度较高的DNA。提取的RNA质量可满足cDNA文库构建,基因芯片探针标定和RT-PCR等对RNA质量要求较高的分子生物学操作,说明这是一种应用范围广的RNA提取方法。
A total RNA extraction method is introduced, the main extraction reagents used in this method are SDS, chloroform and Tris-phenol, and the reagents of RNA precipitation are LiCl and ethanol. The total RNAs were successfully extracted from Tamarix androssowii （woody plant） , PuccineUia tenuiflora （herbage）, Saperda populnea （insect）, Saccharomyces cerevisiae （fungi） and White-rot fungi by using this method. The total RNA extracted by this method produced clear bands, and the ratios of A260/ A280 were above 1.8. The analysis of RNA precipitation efficiency showed that total RNA could be deposited completely in 10 minutes by the cooperation of LiCl and ethanol. At the same time, high purity DNA also can be isolated by this method, the quality of extracted RNA can meet the need of some molecule biology work requiring high quality RNA, such as cDNA library construction, labeling probes for gene chip and RT-PCR. The above described shows this method for RNA isolation can be widely applied.
出处
《植物研究》
CAS
CSCD
北大核心
2006年第1期84-87,共4页
Bulletin of Botanical Research
基金
国家973计划资助项目(G1999016000)
国家转基因植物研究与产业化专项(JY03A1802)
黑龙江省攻关项目(GB02B105)资助
关键词
RNA
提取
方法
RNA
extraction
method
