摘要
目的探讨丙戊酸(VPA)对脊髓损伤后脑源性神经营养因子(BDNF)和神经营养素3(NT-3)表达的影响。方法将60只雄性SD大鼠随机分为三组:对照组(C组)、损伤组(SCI组)和丙戊酸保护组(VPA组)。采用改良Allen法制作脊髓损伤动物模型。VPA组术后即刻及其后每12 h皮下注射VPA 300 mg/kg;C组和SCI组在相应时间点注射等体积的生理盐水。于伤后24、48、72 h和1周取材。利用BBB评分标准进行不同时段的行为学评分。通过免疫组化法观察各组大鼠脊髓损伤后BDNF及NT-3表达的变化。结果 BBB评分显示,C组运动功能未受影响,VPA组的BBB评分均高于SCI组,在伤后48、72 h和1周两者比较,差异有统计学意义(P<0.05)。与C组比较,SCI组和VPA组的BDNF及NT-3表达在各时间点均明显增加(P<0.05),但VPA组各时间点的BDNF及NT-3表达均明显高于SCI组(P<0.05)。结论 VPA可促进大鼠脊髓损伤后神经功能恢复,其机制可能与促进BDNF及NT-3表达有关。
Objective To investigate the effects of valproic acid（VPA） on the expression of BDNF and NT-3 in rats after acute spinal cord injury.Methods 60 adult rats were randomly divided into three groups： control group（C group）,spinal cord injury group（SCI group） and VPA treatment group（VPA group）.Spinal cord injury model was made by modified Allen technique.VPA（300 mg/kg） was administrated in rats through subcutaneous injection immediately after injury and repeated per 12h after injury,while C group and SCI group were received the same dose of normal saline injection at the same time point.The rats were killed at 24,48,72 h and 1 week after injury.The behavior scores were evaluated with BBB scale at each time point after injury.The expression of BDNF and NT-3 were detected by immunohistochemistry method.Results The BBB score indicated that motor function in the C group was normal,and the scores of the VPA group were significantly higher than those of the SCI group at 48,72 h and 1 week after injury,the differences were statistically significant（P 〈0.05）.Compared with C group,the expression of BDNF and NT-3 both increased obviously at each time point after injury in the VPA group and SCI group（P 〈0.05）,however,compared with the SCI group,the expression of BDNF and NT-3 evidently increased in the VPA group at each time point after injury（P 〈0.05）.Conclusion VPA can promote neurofunctional recovery after spinal cord injury through inducing high expression of BDNF and NT-3.
出处
《中国医药导报》
CAS
2012年第21期23-25,共3页
China Medical Herald
基金
四川省成都医学院自然科学基金(基金编号:CYZ07-014)
关键词
脊髓损伤
丙戊酸
脑源性神经营养因子
神经营养素3
Spinal cord injury
Valproic acid
Brain derived neurotrophic factor
Neurotrophin-3
