摘要
目的 建立中药材独活中蛇床子素和异欧前胡素的RP-HPLC含量测定方法。方法 用6倍量体积的甲醇、40℃水浴下提取2次，每次2h，采用Thermo C18柱（250mm×4．6mm，5μm），流动相为甲醇-体积分数为3％的乙酸水溶液（体积比为70：30），流速为1．0mL·min^-1，检测波长为307nm。结果 理论塔板数以蛇床子素和异欧前胡素计算均不低于24000，两物质的色谱峰之间及与相邻峰之间分离度均大于1．5，对称因子均在0．95～1．05之间。蛇床子素和异欧前胡素的线性范围分别为29．9～180mg·L^-1和1．51～18．1mg·L^-1，平均回收率为104．3％和100．0％。蛇床子素和异欧前胡素的精密度和重复性实验结果分别为0．16％和0．85％、1．3％和2．0％。稳定性试验表明，样品溶液中2种成分在24h内稳定性良好。结论 建立的方法灵敏、准确、重现性好，可用于中药材独活的质量控制。
Objective To establish a reversed-phase HPLC method for determination of osthole and isoimperatorinin Angelica pubescens. Methods The sample was separated on a Thermo C18 column（250 mm × 4.6 mm, 5μm）using methanol-3 % acetic acid solution（ V： V = 70：30）as the mobile phase at a flow rate of 1.0 mL＇ min-1 The detection was set at 307 nm. Results The calibration curve was linear in the range of 29.9 - 180.0 mg·L^- 1 （ r = 0.999 9）for osthole and 1.51 - 18.1 mg·L^- 1 for isoimperatorin（ r = 1. 000）. The inner-day RSD was 0.16% and 0.85%, and the average was 104.3% and 100.0% for osthole and isoimperatorin, respectively. It was stable within 24 hours. Conclusions This method is accurate, simple and sensitive, and it can be used for the quality control of Angelica pubescens.
出处
《沈阳药科大学学报》
CAS
CSCD
北大核心
2006年第2期93-96,共4页
Journal of Shenyang Pharmaceutical University
