摘要
从铁胁迫诱导的拟南芥植株根中克隆了全长的FR 02(Fe3+-螯合物还原酶)基因,并将FR 02基因构建到了植物高效表达载体pCB 302中。通过农杆菌介导的转化和PCR及Southern b lot检测证明FR 02基因正向的插入了苹果基因组中。以破坏生长点的茎尖为外植体的苹果基因转化方法转化率高达10.8%。FR 02全长基因的成功克隆、构建和转化为高铁、抗黄化苹果和其他高铁作物新品种的培育开辟了一条新路。
The full-length gene containing restriction enzyme sites was cloned from the roots of Arabidopsis grown under Fe^3＋ -deficient conditions. The gene was inserted into the expression vector-pCB 302 and transformed into Agrobacterium C 58 C 1. After the gene was transferred to apple plants by Agrobacterium-mediated transformation, PCR and southern blotting were performed to analyze the result of the insertion of the FR 02 gene into the gcnomic DNA of the apple cells. The gene transformation method of apple with broken stem tip proved to be efficient with a transformation rate of 10.8%.
出处
《西南农业大学学报（自然科学版）》
CSCD
北大核心
2005年第6期777-780,共4页
Journal of Southwest Agricultural University
基金
天津市自然科学基金资助项目(023614211)
关键词
FR
02基因
基因克隆和构建
缺铁黄化
基因转化
苹果
FR 02 gene
cloning construction of expression vector
Fe - deficient chlomsis
transformation
apple
