摘要
研究了固相萃取-高效液相色谱法测定痕量7种生物胺(色胺、酪胺、组胺、腐胺、尸胺、精脒、精胺)的方法。7种不同生物胺的标样混合溶液与荧光衍生剂丹酰氯在特定条件下衍生,衍生后通过C18固相萃取柱净化、富集,InertsilODS-3色谱柱分离,荧光检测器检测,流动相为乙腈和水梯度洗脱20min,流速为1mL/min。变异系数(CV%)为0.51～2.23,检测低限为1.29×10-6～2.57×10-4μg/mL。
The method for determination of seven minim biogenic amines （tyramine, tyramme, histamine, putrescine, cadaverine, spermidine, spermine） by solid phase extraction-high performance liquid chromatography is reported. Pre-column dansylation of the biogenic amines and subsequent solid-phase extraction of the derivatives through C18 cartridges. Then, dansyl derivatives are separated on Inertsil ODS-3 column using a 20-min gradient elution with a binary system of acetonitrile-water, a flow-rate of 1 mL min^-1.They are detected by fluorescent detector.The variation coefficients are 0.51%-2.23 %, the detection limit is 1.29×10^-62.57×10^-4 μg/mL.
出处
《食品研究与开发》
CAS
北大核心
2006年第1期107-111,共5页
Food Research and Development
