摘要
In order to conduct the fundamental investigation of the effect of 5-FU on HNE1 cells,p33ING1 mRNA level in cellular total RNA was detected quantitatively based on MB assay.During the operation,the effects of 5-FU concentrations and treatment time in HNE1 cells and HNE1 cells transfected with p33ING1 were measured in vitro.The results were as follows: p33ING1 mRNA expression level in tumor cells was enhanced not only by the 5-FU concentration but also by lapse of time.The MTT results also proved that high expression of p33ING1 mRNA can increase cell’s sensitivity to chemical drug 5-FU.The detection method based on MB can be used to provide useful evidence quickly and quantitatively for gene expression and new chemical drugs development.
In order to conduct the fundamental investigation of the effect of 5-FU on HNE1 cells, p33^ING1 mRNA level in cellular total RNA was detected quantitatively based on MB assay. During the operation, the effects of 5-FU concentrations and treatment time in HNE1 cells and HNE1 cells transfected with p33^ING1 were measured in vitro. The results were as follows： p33^ING1 mRNA expression level in tumor cells was enhanced not only by the 5-FU concentration but also by lapse of time. The MTr results also proved that high expression of p33^ING1 mRNA can increase cellg sensitivity to chemical drug 5-FU. The detection method based on MB can be used to provide useful evidence quickly and quantitatively for gene expression and new chemical drugs development.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2006年第2期268-270,共3页
Chemical Journal of Chinese Universities
基金
国家重点基础研究发展规划(批准号:2002CB513110)
国家自然科学基金重点项目(批准号:20135010)
国家自然科学基金青年基金(批准号:20305006)
湖南省科研计划重点项目(批准号:0399Y1006)资助
