摘要
本文介绍锌和邻二氮菲－溴甲酚绿在氨性缓冲液中形成三元配合物增溶于聚乙烯醇ＰＶＡ＿（１２４）体系，直接在水相中测其吸光度。配合物最大吸收位于６１２ｎｍ处，表观摩尔吸光系数ε＿（６１２）＝１．６３×１０￣６Ｌ·ｍｏｌ￣（－１）·ｃｍ￣（－１）。锌浓度在０－１．２μｇ／２５ｍＬ范围内与吸光度呈线性关系。用此法分析良／恶性肿瘤细胞切片中锌含量，获得良好的结果。
A highly sensitive spectrophotometric method based on the formation of tribasic complex of zinc ion with ohenanthroline-bromocresol green has been developed for the determination of micromounts zine. The maxium absorption wavelength of the complex is 612nm and its apparent molar absorptivity is 1. 63×10￣6L·mol￣(-1) . cm￣(-1). Beer 's law is obeyed for zinc in the rang of 0-1.2μg/25mL. The method has been applied to the determination of zinc in tumor with satisfactory results .
出处
《光谱实验室》
CAS
CSCD
1995年第2期57-59,共3页
Chinese Journal of Spectroscopy Laboratory
关键词
锌
邻二氮菲
配合物
分光光度法
肿瘤
Zinc
Phenanthroline
Tribasic Complex
Spectrophotometry.
