摘要
谷物样品中各单糖及游离糖、淀粉、可溶性和不溶性ＮＳＰ流水序列测定方法：用８０％乙醇提取游离糖；而后用乙酸缓冲液、α－淀粉酶和葡萄糖苷酶分解淀粉，得到可溶和不可溶ＮＳＰ；用葡萄糖氧化酶／过氧化酶方法测定淀粉；ＮＳＰ和游离糖再经水解。与内标共同完成还原和酰化过程，用装有高级性毛细管柱的ＧＣ测定。该法不仅可给出构成ＮＳＰ各单糖含量，也可给出淀粉和游离糖的量值。
A stream-lined assay procedure has been developed for the determination of monosaccharides, free sugars,starch, soluble and insoluble polysaccharides in cereal and cereal products on a single-sample basis. The sample is extracted with 80% ethanol to remove free sugars, and then treated with highly specific α-amylase and amyloglucosidase (in acetate buffer at pH 5. 0)to degrade starch and to fractionate the soluble and insoluble non-starch polysaccharides (NSP). An aliquot of the glucose-containing supernatant is taken and analyzed for starch ,using the glucose oxidase/peroxidase method. The free sugars, the soluble and insoluble fractions of NSP are hydrolyzed by acids, and the released monosaccharides are reduced and acetylated along with a known amount of internal standard. The resulting alditol acetates are determined by GC with a polar capillary column. The proposed method can determine not only the monosaccharide constituents of NSP ,but the starch and free sugars as well.
出处
《分析测试学报》
CAS
CSCD
1996年第3期22-25,共4页
Journal of Instrumental Analysis
