摘要
[目的]研究细胞内谷胱甘肽(GSH)对砷致人角质形成细胞系(HaCaT)氧化损伤的保护作用。[方法]用流式细胞仪检测细胞内二氯荧光素(DCF)的荧光强度;用改良硫代巴比妥酸荧光法测定细胞内丙二醛(MDA)含量。[结果]单独用NaAsO2作用后,DCF荧光强度和MDA含量与对照组比较差异有显著性意义(P<0.05),用N-乙酰半胱氨酸(NAC)预处理后,细胞内DCF荧光强度和MDA含量与砷作用组相比差异有显著性意义(P<0.05),其中MDA达到对照组水平。用丁硫氨酸亚矾胺(BSO)预处理细胞后,DCF荧光强度和MDA含量与NaAsO2单独作用组相比明显增高(P<0.05)。[结论]NAC可减轻砷对细胞的氧化损伤,而BSO则可加重其氧化损伤,说明细胞内的GSH可对砷引起的氧化损伤起一定的保护作用。
[ Objective] To study the protective effect of GSH on oxidative damage in HaCaT cells induced by arsenic. [ Methods ] The fluorescence intensity of DCF was detected by flow cytometry. The MDA content was detected by improved thio - barbituric acid fluorometric method. [ Results ] The fluorescence intensity of DCF and the MDA content increased significantly （P 〈 0.05） in 5 μmol/L NaAsO2 group. In the group of pre - treating with NAC, the fluorescence intensity of DCF and the MDA content decreased significantly （P 〈 0.05）. While in the group of pre - treating with BSO, the fluorescence intensity of DCF and the MDA content increased significantly （ P 〈 0.05 ）. [ Conclusions ] NAC can ameliorate the oxidative damage induced by arsenic, while BSO aggravates the oxidative damage. These results indicate that GSH Has protective against the oxidative damage of cells exposed to arsenic.
出处
《大连医科大学学报》
CAS
2008年第6期500-502,共3页
Journal of Dalian Medical University
基金
国家自然科学基金(30600488
30571584)
辽宁省教育厅课题(20081070)
大连市科技基金(2007J23JH037)
