摘要
采用传统方法对15株新城疫分离毒株进行毒力测定,根据鸡胚平均死亡时间(MDT)与3日龄雏鸡脑内接种指数(ICPI)测定结果,FW、F48E8属于速发型毒株;ND98、P1、P2,鸽NDV、Ⅰ系、H2、BY属于中发型毒株,Ⅱ系、L2、P3、ND99,ND89,Lasota属于缓发型毒株。同时对这些毒株的表型特征进行观察发现,病毒血凝解脱速率、血凝素对热的稳定性、对不同动物红细胞的血凝谱等表型特征与NDV毒力没有相关性。通过间接酶联免疫吸附试验,采用针对NDV HN基因的单克隆抗体对贵州ND不同分离株进行分群研究,表明A群为速发型强毒株;B群为缓发型弱毒与某些中等毒力株;C群分离物的毒力介于速发型强毒与中等毒力之间。
The virulence of fifteen NDV strains was identified by the traditional methods. According to the results of MDT（mean death time） and ICPI （intraeerebral pathogenicity index）, FW and F48 E8 were classified into velogenie strains; ND98, P1, P2, NDV, I , H2 and BY belonged to mesogenie strains; Ⅱ, L2 , P3, ND99, ND89 and Lasota were lentogenie strains. At the same time, three phenotypie characteristics of these virus were determined, including hemagglutinin elution rate , heat-stability and hemagglutination spectrum with red blood cells of different animals. No relativity was existed between three phenotypie characteristics and virus virulence. The indirect enzyme linked immunosorbent assay （ELISA） was conducted and all the tested strains were divided into three groups by monoelonal antibodies which directed against HN glyeoprotein of NDV. The results indicated that group A was velogenie, group B included mesogenie and some lentogenie strains, group C consisted of the epidemic strains in the field, the virulence was intermediate between the velogenie and mesogenie strains.
出处
《山地农业生物学报》
2005年第6期491-496,共6页
Journal of Mountain Agriculture and Biology
关键词
新城疫
毒力测定
单克隆抗体
分群
Newcastle disease
virulence
monoclonal antibody
grouping
