摘要
应用RT-PCR技术从新城疫病毒洛阳分离株中扩增HN的cDNA片段，并将其克隆至pMD18-T载体进行核苷酸序列测定。结果表明：新城疫分离株HN基因片段长度为1716bp，编码571个氨基酸；推测出的氨基酸序列中有5个糖基化位点，12个半胱氨酸残基；与国内外的14株新城疫毒株HN基因相比，核苷酸和氨基酸同源性分别为66．4％～98．5％和87．8％～98．3％；与标准毒株Lasota和Clone30的亲缘关系较远，核苷酸和氨基酸差异性较大，而与天津分离株和云南分离株的亲缘关系较近，基本属于同一基因群。
The cDNA fragments of HN gene of Newcastle disease virus（NDV） from Luoyang were amplified by RT - PCR and cloned into pMD18 - T vector, the recombinant plasmids were sequenced. The results showed that the HN gene fragments consisted of 1 716bp coding for 571 amino acids which contained 5 glycosylation sites and 12 cysteine residues. Compared with the other 14 Newcastle disease virus strains discovered, the sequence homology of neucletide and amino acids were from 66.4% to 98.5% and from 87.8% to 98.3%, respectively. The virus insolated from Luoyang had a further distance with the standard virus lasota and cloning 30, and was closer with the virus strains from Tianjin and Yunnan.
出处
《河南农业科学》
CSCD
北大核心
2006年第12期82-86,共5页
Journal of Henan Agricultural Sciences
