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不同新城疫病毒株结构蛋白的分析 被引量：3

The structural proteins analysis of different virus strains of newcastle disease
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摘要 2株新城疫病毒ND98、ND99分别分离自发病鸡群与雏鸵鸟,ND98属于中发型毒株,ND99属于缓发型毒株。将2株分离株ND98、ND99与参考毒株F48E8、Lasota进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)、蛋白质转移印迹(Western-Blotting)。SDS-PAGE结果显示,ND98、ND99与F48E8、Lasota具有相似的结构蛋白:HN蛋白、F蛋白、NP蛋白与P蛋白、M蛋白;Western-Blotting试验显示,ND98与F48E8、ND99与Lasota具有相似的免疫原,ND98、ND99免疫原性存在一定的差异。 Two newcastle disease viruses(NDV) isolated from chickens and ostriches were designated as ND_(98) and ND_(99) respectively.ND_(99) was lentogenic strain and ND_(98) was mesogenic strain.The structural proteins of ND_(98)、ND_(99)、F_(48)E_8、Lasota stains were analyzed by SDS-PAGE and Western-Blotting.The result indicated that ND_(98)、ND_(99) strains had the same structural proteins as Lasota and F_(48)E_8 strains.Western-Blotting test suggested that antigen of ND_(98) and ND_(99) existed some difference,ND_(98) and F_(48)E_8 strains shared the same immunogen as ND_(99) and Lasota strains.
作者 温贵兰 周碧君
机构地区 贵州大学动物科学学院
出处 《山地农业生物学报》 2005年第2期124-127,共4页 Journal of Mountain Agriculture and Biology
基金 贵州省科学技术基金资助项目[黔科合农社字(1998)3086]
关键词 新城疫病毒株 结构蛋白 新城疫 免疫原 聚丙烯酰胺凝胶电泳 蛋白质转移印迹 newcastle disease virus SDS-PAGE Western-Blotting structural protein immunogen
分类号 S852.659.5 [农业科学—基础兽医学]
  • 相关文献

参考文献6

二级参考文献22

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共引文献24

同被引文献18

  • 1瞿继红,周碧君,温贵兰,汪德生,彭秋.不同新城疫分离株的毒力测定与单克隆抗体分群研究[J].山地农业生物学报,2005,24(6):491-496. 被引量：4
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  • 3Lomniczi B, Wehmann E, Herczeg J, et al. Newcastledisease outbreaks in recent years in Western Europewere caused by an old (Ⅵ) and a novel genotype (Ⅶ) [J]. Arch Virol,1998,143:49-64.
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  • 5Li Y, Wang Z, Jiang Y, et al. Characterization of newly emerging Newcastle disease virus isolates from the People's Republic of China and Taiwan[J]. Clin Microbiol, 2001,39 : 3512-3519.
  • 6HERCZEG J, WEHMANN E, BARGG R R, et al. Two novel genetic groups ( Ⅶ b and Ⅷ ) responsible for recent Newcastle disease outbreaks in Southern Africa, one (VIIb) of which reached Southern Europe [ J ]. Arch Virol, 1999, 144 ( 11 ) : 2087 - 2099.
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  • 10Lornniczi B, Wehmann E, Herczeg J, et al. Newcastledisease out breaks in recent years in Western Europewere caused by an old ( Ⅵ ) and a novel genotype ( Ⅶ ) [ J ]. Arch Virol, 1998, 143 : 49 -64.

引证文献3

二级引证文献2

山地农业生物学报
2005年 第2期

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