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细胞外信号调解激酶对蟾蜍灵诱导白血病细胞凋亡影响的研究 被引量：1

Effect of extracellular signal-regulated kinase on the apoptosis induced by bufalin in K562 cells
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摘要 目的:探讨细胞外信号调解激酶(ERK)对蟾蜍灵诱导白血病细胞凋亡的影响。方法:形态学观察和流式细胞仪检测细胞凋亡。结果:0.5μmol/L蟾蜍灵作用K562细胞24 h诱导典型的细胞凋亡,预先用ERK抑制剂PD98059处理细胞1 h,明显增加蟾蜍灵诱导的细胞凋亡率(P<0.05);而用佛波酯预先处理后,明显拮抗蟾蜍灵的作用(P<0.05)。结论:ERK负向调节蟾蜍灵诱导的K562细胞凋亡过程。 Objective： To explore the effect of extraceUular signal-regulated kinase （ERK） on the apoptosis induced by bufalin in K562 ceils. Methods： Apoptosis was determined by morphological method and flow cytometry. Results： Bufalin of 0.5 μmol/L induced the apoptosis of K562 ceils at 24 hours, and the action of bufalin was enhanced by ERK inhibitor PD98059 and reduced by 12-O-tetradecanoylphorbol-13-acetate. Conclusion： ERK negatively regulated the apoptosis induced by bufalin in K562 cells.
作者 王甦 张晓晶 刘云鹏 侯科佐 罗颖 刘世洲 王妍
机构地区 中国医科大学附属第一医院肿瘤内科 中国医科大学附属第一医院肿瘤外科
出处 《中国医科大学学报》 CAS CSCD 北大核心 2005年第6期503-504,共2页 Journal of China Medical University
基金 辽宁省自然科学基金资助项目(20022077) 辽宁省教育厅基金资助项目(20122152)
关键词 蟾蜍灵 凋亡 白血病细胞 细胞外信号调解激酶 bufalin apoptosis leukemia cells extracellular signal-regulated kinase
分类号 R733.7 [医药卫生—肿瘤]
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参考文献5

  • 1Masuda Y,Kawazoe N,Nakajo S,et al.Bufalin induces apoptosis and influences the expression of apoptosis-related genes in human leukemia cells[J].Leuk Res,1995,19 (8):549-556.
  • 2刘云鹏,曲秀娟,王萍萍,田昕,罗颖,刘世洲,卢香兰.蟾蜍灵诱导K562细胞分化和凋亡过程中WT1表达的下调[J].中华血液学杂志,2002,23(7):356-359. 被引量：52
  • 3Yu CR,Krystal G,Varticovksi L,etal.Pharmacologic mitogenactivated protein/extracellular signal-regulated kinase kinase /mitogen-activated protein kinase inhibitors interact synergistically with STI571 to induce apoptosis in bcr/abl-expressing human leukemia cells[J].Cancer Res,2002,62(1):188-199.
  • 4Racke FK,Lewandowska K,Goueli S,et al.Sustained Activation of the extracellular signal-regulated kinase/mitogen-activated protein kinase pathway is required for megakaryocytic differentiation ofK562 cells[J].J Biol Chem,1997,272(37):23366-23370.
  • 5Keeshan K,Cotter TG,McKenna SL.High bcr-abl expression prevents the translocation of Bax and bad to the mitochondrion[J].Leukemia,2002,16(9):1725-1734.

二级参考文献7

  • 1Algar EN,Khromykh T,Smith SI,et al.A WT1 antisense oligonucleotide inhibits proliferation and induces apoptosis in myeloid leukaemia cell lines[].Oncegene.1996
  • 2Kurosawa M,Tani Y,Nishimura S,et al.Distinct PKC isozymes regulate bufalin-induced differentiation and apoptosis in human monocytic cells[].American Journal of Physiology Cell Physiology.2001
  • 3Zhang L,Nakaya K,Yoshida T,et al.Bufalin as a potent inducer of differentiation of human myeloid leukemia cells[].Biochemical and Biophysical Research Communications.1991
  • 4Svedberg S,Chylicki K,Gullberg U.Downregulation of Wilms’tumor gene (WT1)is not a prerequisite for erythroid or megakaryocytic differentiation of the leukemic cell line K562[].Experimental Hematology.1999
  • 5Yamada K,Hino K,Tomoyasu S,et al.Enhancement by bufalin of retinoic acid-induced differentiation of acute promyelocytic leukemia cells in primary culture[].Leukemia Research.1998
  • 6Numazawa S,Shinoki MA,Ito H,et al.Involvement of Na+ , K+ATPase inhibition in K562 cell differentiation induced by bufalin[].Journal of Cellular Physiology.1994
  • 7Tamaki H,Ogawa H,Inoue K,et al.Increased expression of the Wilms tumor gene(WT1)at relapse in acute leukemia[].Blood.1996

共引文献51

同被引文献23

引证文献1

二级引证文献13

中国医科大学学报
2005年 第6期

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