摘要
目的观察蟾酥胶囊对人肝癌细胞凋亡的诱导作用。方法采用中药血清药理学方法,选择不同浓度含药血清作为实验组,分别与人肝癌BEL-7402细胞共同孵育24h、48h,流式细胞仪检测细胞凋亡率、细胞周期变化及Apo2.7蛋白的阳性表达率;均与10%无药血清组做对照。结果流式细胞仪检测可见,各实验组细胞G1、S期下降,G2/M期升高,产生凋亡峰(sub-G1期),细胞凋亡率与作用时间、含药血清浓度有一定的时效和量效关系。结论蟾酥胶囊含药血清可以诱导人肝癌细胞BEL-7402凋亡,提示蟾酥胶囊是一种有潜力的治疗肝癌药物。
Objective To observe the efficiency of Chansu Capsule with medicated serum in inducing the apoptosis of human hepatocellular carcinoma（HCC） cell. Methods Using serum pharmacologic method of the traditional Chinese medicine, we chose different concentrations of medicated serum containing chansu capsule as study groups, which were respectively co-cultivated with human hepatocellular carcinoma cell BEL-7402 for 24 or 48 hours. The percentage of cell apoptosis, the cycle change of DNA were examined by flow cytometry ;blank serum was taken as control group. Results In study groups,G1 .S of cell cycle decreased, G2/M of cell cycle increased, and the sub-G1 peaks were observed by flow cytomeric assays; the percentage of cell apoptosis increased with the enhancing of time and concentration, with obvious time-response and dose-response relationship. Conclusion The chansu capsule could induce apoptosis of human hepatocellular carcinoma cell BEL7402 and probably become a potential drug of anti-tumor.
出处
《四川解剖学杂志》
2005年第3期9-12,共4页
Sichuan Journal of Anatomy
关键词
蟾酥胶囊
诱导
肝癌
凋亡
Chansu Capsule
induce
HCC
apoptosis
