摘要
阿糖胞耷（ＡＲＡ）能抑制ＤＮＡ切除修复作用，从而使Ｇ０期淋巴细胞的ＤＮＡ初始损害得以固定和表达，并最终形成微核（ＭＮ）．本实验用微量全血培养法，对人淋巴细胞进行了加与不加ＡＲＡ（＋ＡＲＡ，－ＡＲＡ）的实验研究。结果本底＋ＡＲＡ组双核细胞ＭＮ率比－ＡＲＡ组增加了６．４倍，ＩＧｙＸ射线平均增加了３．３倍，ＩＧｖγ射线组平均增加３．５倍，而紫外线组平均增加了６．３倍。结果证明ＡＲＡ可显著增强ＣＢ－ＭＮＴ法对ＤＮＡ小损伤的敏感性，微量全血培养法同样适用于该方法。
It was already found that cytosine arabinoside(ARA)can inhibite the activities ofDNA polymerisate enzyme,thereby the lesions in G0 phase can be fixed and expressed as mi-crOnuclei(MN). The whole blood culture method was used in this study. The production ofMN in the human lymphocytes with and without ARA were compared. We observed thebaseline(no mutagen) of MN frequencies(‰)in +ARA group is increased 6.4-fold of thatin-ARA group,3.3-fold for IGy x-rays,3.5-fold for IGy γ-rays,and 6.3-fold forUItraviolet Light. These observations suggested that combined ARA-CBMN method mayenhance the sensitivities of exposure to genotoxic agents that predominantly induce DNA ex-cision repairable lesions.
出处
《癌变·畸变·突变》
CAS
CSCD
1996年第3期144-149,共6页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
全军青年基金
