摘要
本研究用中低剂量γ射线体外照射4个供血员的全血,系统地比较观察了胞质分裂阻滞与常规微核测试法测得的剂量效应关系,结果表明用常规法检测,低剂量γ-射线(0.1,0.3Gy)可诱发平均MNCF的逐渐增加,和对照组相比,至0.3Gy差异显著(p<0.01);而BC法测得的平均MNCF无上升,相反在0.1Gy时明显下降(p<0.05)。在中剂量区(0.7—3.1Gy),用BC法测得的平均MNCF的增加较常规法明显,至3.1Gy时两者差异显著。
In this paper the authors have studied relationship between γ-rzy dose and mean MNCF measured separately by Cytokinesis-block and conventional micronucleus test (BC- and C-MNT) to increase the sensitivity of MNT in human lymphocyte and to explore the possibility of using MNT in human lymphocyte as biodosimetry. The main results show that using C-MNT low dose of y-rays (0.1, 0.3 GYs) could increase mean MNCF as compared with control; but using BC-MNT mean MNCF could not be increased. With mediun dose of γ-rays (0.7-3.1 GYs) mean MNCF measured by BC-MNT is more significant than that measured by C-MNCT.
关键词
微核
人淋巴细胞
胞质分裂阻滞
Micronuclei, Human lymphocyte, cytokinesis-block method, Conventional method, γ-ray
