摘要
本研究建立的测定纤溶酶原激活物（Ｐｌａ）的纤维蛋白溶解圈法，不仅简单易行，而且具有很高的敏感性和特异性。检测灵敏度较原来的普通溶解圈法提高了大约５００倍，能检出２．５×１０－５ＩＵ的尿激酶（ＵＫ）活力。本方法特别适用于低Ｐｌａ含重的定性定量检测，如革兰氏阴性菌纤溶酶原激活物的测定。应用此方法，本研究首次证明了伤寒和鼠伤寒杆菌具有纤溶酶原激活物的活性，１０Ｄ鼠伤寒菌的Ｐｌａ活性相当于４×１０－２ＩＵＵＫ；１ＯＤ伤寒菌的Ｐｌａ活性相当于５×１０－３ＩＵＵＫ。
Afibrin film lysis zone assay without agarrose gel for plasminogen activator (Pla) has been established.It is highly specific and sensitive,and can detect o.s low as 2.5×10-5IU of urokinase(UK)activity which is 500 times lower than orginal method.it is simple,easy to manipulate and especially suitable for detecting the,amples of low Pla activities.Using this assay,the plasminogen activator activites in G-bacteria had been examined at the first time,and the result clearly showed that S.typhimurium and S.typhi possessed the characteristic of Pla activites:1 OD of S.typhimurium contained about 4×10-2IU of UK; and 1 OD of S.typhi about 5×1O-3IU of UK.
出处
《中国人兽共患病杂志》
CSCD
北大核心
1996年第4期7-10,共4页
Chinese Journal of Zoonoses
基金
国家自然科学基金
