摘要
组织型纤溶酶原激活物（ｔ—ｐＡ）是反映人体纤溶系统活性的一个重要成分，人血浆ｔ—ＰＡ活性的变化与临床许多疾病的发生和发展有密切关系。笔者建立了测定血浆ｔ—ＰＡ活性的酶联纤维蛋肉溶解法，并对该方法的准确度、精密度和灵敏度进行了研究。运用本法确立了不同年龄组的正常参考值，检测了４０例患者血浆ｔ—ＰＡ活性的变化并对其变化的机理作了初步探讨。
An enzyme linked fibrinolytic assay (ELFA) for the measurement of tissue-type plasminogen activator in plasma,using enzyme labelled fibrin, has been established.This assay is sensitive(0.002 5IU /ml),accurate(recovery range from 97 to 110%)und reproducible(CV of intra-assay is 3.6%,in-ter-assay is 4.5%). Using this method,the plasma t-PA activity was assayed in 80 cases of healthy indi-viduals and 40 cases ofpatients, and the results were discussed briefly.
关键词
纤溶酶原
激活物
酶联纤维蛋白
Plasminogen activator, tissue-type Myocarial infarction Diabetes mellitus
