摘要
目的分析颌面部VX2肿瘤DCE-MRI动态曲线与肿瘤乏氧染色和血管内皮生长因子(VEGF)表达水平的相关性,探讨DCE-MRI在肿瘤乏氧评价中的价值。方法8只新西兰大白兔于单侧颌面部咬肌内接种VX2肿瘤细胞0.5ml,接种后10天开始,每隔2天取一只荷瘤兔行DCE-MRI检查,绘制肿瘤动态增强曲线,计算最大强化率(MER)和强化率斜率(SLE)值。对处死的荷瘤兔,取对应层面病理标本行HE染色、乏氧染色和VEGF染色分析。结果8只实验兔成瘤率达100%,肿瘤位于单侧咬肌内,呈实质性,边缘较清晰;肿瘤MER值与乏氧程度行Spearman's等级相关分析存在显著负相关(P<0.01);肿瘤SLE值与VEGF表达水平存在正相关(P<0.05)。对肿瘤乏氧染色及VEGF表达行连续切片对照研究,发现乏氧染色阳性的区域,VEGF表达也相对较高;但VEGF表达阳性区并不完全与肿瘤乏氧区表达一致。结论肿瘤乏氧与VEGF表达关系密切,乏氧可诱导VEGF表达的增高,而VEGF表达能促进肿瘤血管生成。DCE-MRI动态增强参数与肿瘤乏氧程度和VEGF表达水平有很好的相关性。
To analyze the correlation of dynamic contrast-enchanced MR imaging （DCE MRI） with tumor hypoxia and vascular endothelial growth factor （VEGF） expression and to evaluate the value of DCE-MRI in tumor hypoxia im aging. Methods Eight New Zealand rabbits were inoculated with 0.5 ml （1 × 10^8/ml） VX2 tumor cells in the maxillofacial masseter. DCE-MRI was performed every 2 days since the 10th day on. The maximal enhancement ratio （MER） and slope of enhancement （SLE） were calculated. After DCE MRI, the rabbit was sacrificed and the corresponding tumor specimen was harvested. HE staining, hypoxia and VEGF immunohistochemical staining were carried out. Results All the 8 rabbits had tumors growing. The masses located in the unilateral masseters, and had relatively clear margins. MER had a negative correlation with tumor hypoxia degree （P〈0.01）. SLE bore a positive correlation with VEGF immunohistochemical score （P〈 0.05）. We compared the continuous sections of hypoxia with VEGF staining of the tumor, and found that where the more hypoxia staining positive, the stronger VEGF expression. But VEGF staining was not always coincident with the staining of hypoxia. Conclusion Tumor hypoxia had a close relationship with the expression of VEGF, and VEGF could promote tumor angiogenesis. DCE MRI had a good correlation with tumor hypoxia degree and VEGF expression.
出处
《中国医学影像技术》
CSCD
北大核心
2005年第11期1644-1647,共4页
Chinese Journal of Medical Imaging Technology
基金
国家自然科学基金资助(30170286)
上海市卫生局科学技术发展基金资助(01436)
关键词
磁共振成像
图像增强
VX2肿瘤
乏氧
血管内皮生长因子
Magnetic resonance imaging~ Image enehaneement
VX2 tumor
Hypoxia
Vascular endothelial growth factor
