摘要
目的:制作兔舌不同部位VX-2鳞癌和颈淋巴结转移模型,并对其生物学特性进行研究,为舌癌多学科治疗提供研究平台。方法:将新西兰白兔20只,随机分为4组,每组5只,分别植入VX-2鳞癌瘤块。观察瘤块植入后肿瘤生长、体积变化、成瘤率、颈淋巴结转移率、转移的部位、数目以及兔生存期等指标。数据采用SPSS11.5软件包进行游程检验、独立样本t检验和χ2检验。结果:总成瘤率75%,其中舌缘80%,舌中线区70%,颈淋巴结转移率均70%,舌缘转移至同侧颈淋巴结,舌中线区转移至双侧颈淋巴结。平均生存期为舌缘(33.25±6.20)d,舌中线区(39.00±3.74)d。各区间无统计学意义(P>0.05),舌癌和颈部转移淋巴结均为低分化鳞癌。结论:瘤块移植法能成功制作不同部位兔舌VX-2鳞癌模型,成瘤率和转移率均较高,生存期为4～6周。其生物学特性与人舌癌基本一致,生长迅速,容易转移,是研究舌癌较理想的动物模型。
PURPOSE： To make a VX-2 squamous cell carcinoma model in different sites of rabbit tongue and cervical lymph node metastasis and study its biological properties, in order to offer a research platform for multidisciplinary therapy of tongue carcinoma. METHODS： Twenty New-Zealand white rabbits were divided into four groups, five in each group, which were implanted with VX-2 squamous cell carcinoma tissue pieces. Tumor growth, volume change, tumor-bearing rate, metastasis rate of cervical lymph node, site and quantity of lymph node metastasis and rabbits＇ survival period were observed and recorded. The data were analyzed by Runs test, independent-samples test and Chi-square test with SPSS11.5 software package. RESULTS： Tumor-bearing rate was 75%, 80% in lateral border of tongue and 70% in median line of tongue. Metastasis rate of cervical lymph node was 70%.Tumors in the lateral border of tongue metastasized to the ipsilateral lymph nodes and that in the median line of the tongue metastasized to bilateral lymph nodes. Rabbits＇ average survival period were （33.25±6.20）days in lateral border of tongue and （39.00±3.74）days in the median line of tongue. Differences in each site were not statistically significant（P〉0.05）. Pathologically, tongue carcinoma and cervical lymph node metastasis were both poorly differentiated squamous cell carcinoma. CONCLUSIONS： With modality of tissue pieces, model of VX-2 squamous cell carcinoma in different sites of rabbit tongue is made successfully. Tumorbearing rate and metastasis rate of cervical lymph nodes were both higher, rabbits＇ survival periods were 4 to 6 weeks. Its biological properties are accordance with human tongue carcinoma.The tumor is growing rapidly and metastasizing easily. So it＇s an ideal animal model to study tongue carcinoma. Supported by Shanghai Leading Academic Discipline Project（Grant No.Y0203）.
出处
《上海口腔医学》
CAS
CSCD
2007年第5期497-501,共5页
Shanghai Journal of Stomatology
基金
上海市重点(优势)学科建设项目(Y0203)
关键词
兔
VX-2鳞癌
动物模型
Rabbit
VX-2 squamous cell carcinoma
Animal model
