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一步免疫印迹法同时检测抗RA33抗体和抗ENA抗体及其对RA患人的诊断价值 被引量：1

The use of one-step immunoblotting to detect both anti-RA33 and anti—ENA antibodies,and its diagnostic value for RA patients
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摘要 目的提高抗RA33抗体诊断类风湿性关节炎(RA)的特异性。方法建立一步免疫印迹法,可同时检测抗RA33抗体和抗ENA抗体。并对266例不同风湿性疾病患者血清和24例正常人血清进行了检测。结果抗RA33抗体阳性率:RA 38%(38/100),SLE 35.8%(24/67),MCTD 36.4%(8/22),SS 8.3%(3/36)。抗RA33抗体阳性血清中抗ENA抗体的检出率:95.8%(23/24)SLE有抗Sm/u1RNP抗体,100%(8/8)MCTD有抗u1RNP抗体,100%(3/3)SS有抗SSA/SSB抗体,RA血清抗ENA抗体均为阴性。结论本文建立的一步免疫印迹法可有效地鉴别抗RA33抗体阳性的RA和SLE、MCTD等病人。 Objective To improve the diagnostic specificity of anti-RA33 antibody for rheumatoid arthfitis（RA）. Methods Establishment of one-step immunoblotting technique to detect both anfi-RA33 and anti-ENA antibodies simultaneously, and 266 sera from different rheumatic patients were detected. Results The positive rate of anfi-RA33 antibody was 38.0% （38/100） in RA, 35.8%（24/67） in SLE, 36.4%（8/22） in MCTD, 8.3%（3/36） in SS respectively. Among these anti-RA33 positive sera, anti-Sm/ulRINP bands were detenninated in 95.8% （23/24） SLE, 100% （8/8） MCTD respectively, and anti-SSA/SSB bands were determi- nated in 100%（3/3） SS sere. In contrast, none of the anfi-ENA bands above were found in RA sere. Conchusion The one-step im- munblotting in this study is extremely useful to serologically differentiate anti-RA33 positive RA from anti-RA33 positive SLE and MCTD patients.
作者 刘学明 于秀明 于雅婷 侯云峰 孙然 田园
机构地区 长春中医学院附属医院
出处 《中国实验诊断学》 2005年第5期754-756,共3页 Chinese Journal of Laboratory Diagnosis
基金 吉林省科技厅科技发展计划项目(吉科合字第970576号)
关键词 类风湿关节炎 RA33抗体 免疫印迹法 ENA抗体 rheumatoid arthritis anti-RA33 antibody immunoblotting anti-ENA antibodies
分类号 R593.22 [医药卫生—内科学]
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中国实验诊断学
2005年 第5期

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