摘要
采用套式聚合酶链反应检测丙肝患者血清，唾液和尿各５２份。结果血清ＨＣＶ－ＲＮＡ阳性３９例，２３例分别在尿和唾液中检出ＨＣＶ－ＲＮＡ；１３份血清ＨＣＶ－ＲＮＡ阴性者中，尿和唾液均未检出ＨＣＶ－ＲＮＡ，有非常显著差异（Ｐ＝０．０００１）。血清ＨＣＶ－ＲＮＡ阳性者分ＡＬＴ正常组和异常组，尿和唾液ＨＣＶ－ＲＮＡ阳检率分别为１８％和７５％，亦有非常显著差异（Ｐ＝０．００１）。对照组１３例正常人血清、唾液和尿ＨＣＶ－ＲＮＡ均为阴性。表明丙肝患者尿和唾液中能否检出ＨＣＶ－ＲＮＡ与病毒血症及ＡＬＴ有关，提示丙肝患者的尿和唾液可能为ＨＣＶ感染的传播媒介。
Fifty-one patients with hepatitis C were detected for HCV-RNA in serum, urine and saliva using nested-PCR. Of 39 patients with positive serum HCV-RNA, 23 were HCV-RMA positive in urine or saliva (14/39 in urine, 9/39 in saliva). Of 13patients with negative serum HCV-RNA,all were HCV-RNA negative in urine or saliva. Thore was a significant difference between them (P=0.0001). Thirty-nine patients with positive serum HCV-RNA were divided into serum ALT normal and abnormal groups. HCV-RNA positive ratea were 18% and 75% in urine or saliva ofpatients from serum ALT normal group and abnormal group respectively.There was aiso a significant difference between the two groups (P=0001).serum, saliva and urine of 13 normal controls were negative for HCV-RNA. It indicates that detection of HCV- RNA in urine and saliva is related with HCV viremia and serum ALT level. These results provide a biological basis for urine and saliva as possible sources of HCV infection, and may imply transmission by these routes.
出处
《上海第二医科大学学报》
CSCD
1996年第3期224-228,共5页
Acta Universitatis Medicinalis Secondae Shanghai
关键词
丙型肝炎
聚合酶链反应
唾液
尿
Hepatits C Virus (HCV)
Nested-PCR
transmission
