摘要
利用水杨酸(SA)诱导烟草抗病相关基因表达,以此为目标样本,以清水喷施为对照样本,进行抑制差减杂交,构建烟草抗病基因表达文库.结果显示,插入片段主要集中在150～500bp之间.随机挑选12个克隆进行测序,结果有7条与已知的系统获得性抗病基因同源,1条与病程相关蛋白PR1a同源,1条与光系统II的促氧蛋白一个亚基有较高同源性,1条与水通道蛋白基因(aquaporin 1)同源,1条与Ert13基因有关,1条未找到同源序列,是新的cDNA片段.
The SSH library of differently expressed cDNA was constructed, in which the tobacco with salicylic acid stimulated as tester, and one with water sprayed as driver. Analysis showed that most of the inserted fragments were 150 - 500 bp. Twelve clones were selected randomly and sequenced. The BLASTN homology analysis on GenBank revealed that seven clones have homology with tobacco resistance genes, one clone is homologous with PR1 a, other three are homologous with oxygen-evolving protein, aquaporin 1 and Ertl3 gene, only one is a new eDNA with no homologous sequence.
出处
《云南农业大学学报》
CAS
CSCD
2005年第6期771-773,共3页
Journal of Yunnan Agricultural University
基金
云南省烟草公司资助项目(03A010515)
