摘要
针对课题在构建烟草cDNA文库的过程中,需要处理大量DNA序列的测序结果和进行相关的序列分析和管理,根据实验的具体要求,采用微软公司新一代的计算机编程语言.NET研究开发相应的管理分析软件,开发出烟草核酸数据库管理系统,软件能完成直接读取相应的测序报告文件,并将结果储存于数据库当中,并且具有远程自动比对功能,对结果进行分析。通过使用该软件系统,完成了对近1千条序列的直接管理和辅助分析,发现了烟草核酸新序列,设计探针以进行分子生物学有关研究,提高了整个实验过程的效率。
In the process of constructing the tobacco DNA library, it often needs to process the massive data of DNA sequencing results, subsequently, carries on the sequence analysis and the management. According to the experience concrete request, the software for the management and analysis has been developed, it adopting .NET language, the new generation calculator language of the Microsoft company, can easily read then sequencing report files, saving the result into the database automatically, and it has the function to distance Blast online. After the direct management and assistance analysis of more than one thousand sequences, new sequences of nucleic acid from tobacco has been found, then, we design the probe to carry on the relevant research of molecular biology by Tobacco Nucleic Acid Database Management System, it enhances the efficiency of the entire experiment.
出处
《中国农学通报》
CSCD
2007年第1期371-375,共5页
Chinese Agricultural Science Bulletin
基金
国家自然科学基金项目"烟草富含甘氨酸RNA结合蛋白分子生物学研究"(30560062)
中国烟草云南省公司科技开发计划项目"云南烟草基因组BAC文库的构建"(03A01)
中国烟草云南省公司科技开发计划项目"烟草品质相关基因表达谱分析及在育种实践中的应用"(05-15)。
关键词
烟草
CDNA文库
核酸序列
数据库
管理系统
Tobacco, cDNA Library, Nuclear Acid Sequence, Database, Management System
