摘要
采用ＰＣＲ－ＲＦＬＰ方法对５４例上海人随机样本作ＨＬＡ－ＤＱＡ１基因分型，其中２４例已有ＰＣＲ－ＳＳＯＰＨ分型结果。通过分析比较，发现这两种方法得出的结论基本一致。
The second exons of HLA-DQA1 genes of Chinese heterozygous were amplified with polymerase chain reaction (PCR ) , followed by digestion of the amplified DNA segments with allele-specific restriction endonuclease. The resulted patterns of restriction fragment length polymorphism (PCR-RFLP ) in polmacrylamide gel electrophoresis were used for HLA-DQA 1 genotyping. With advantages such as saving time , accuracy and no need for radio isotope to label oligonucleotide probes for hybridization , the technique has been proved to be capable of genotyping Chinese heterozygous cells. ln this paper , 24 of 54 samples tested with PCR-RFLP method have been typed with PCR-SSOPH method . The differences between two methods were analysed
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
1996年第1期68-72,共5页
Chinese Journal of Microbiology and Immunology
