摘要
目的研究黄芪提取物(EA)对大鼠全脑缺血再灌注炎症损伤的保护作用。方法采用大鼠四动脉结扎模型,观察EA对大鼠缺血再灌注大鼠神经功能学评分、血清IL-1β、IL-6、TNF-α含量及脑组织IL-1β、TNF-α的含量的影响。结果EA对全脑缺血再灌注24h后的大鼠神经功能有改善作用;EA能降低大鼠全脑缺血再灌注后血清IL-1β、TNF-α、IL-6和脑组织IL1-β、TNF-α的含量。结论EA对大鼠全脑缺血再灌注损伤有一定的保护作用,其机制可能与抑制IL-1β、TNF-α、IL-6的表达有关。
Objective To explore the protective effects of extract of astragalus on inflammatory injury after global cerebral ischemia/reperfusion in rats. Methods The model of global cerebral ischemia/reperfusion was established with four vessel occlusion method（4-VO）. Sprague-Daewley rats were randomly divided into therapy groups, shame operation group and ischemia/reperfution group. Vertical screen test and beam balance test were used to evaluate the rats＇ behavioral function at 12 h and 24 h after reperfusion. After global cerebral ischemia/reperfusion, the rats＇ serum and cortex IL-1β, IL-6 and TNF-α were detected with radio -immuno-assay. Results Compared with ischemia/reperfusion group, serum and cortex IL-1β, IL-6, TNF-α of EA （40,80 mg/kg） group declined significantly after global cerebral ischemia/reperfusion. Behavioral function of EA group after 24 h reperfusion was improved to some extent. Conclusion EA has protective effects on cerebral ischemia which is related to inhibition of cytokine expression.
出处
《安徽医科大学学报》
CAS
北大核心
2005年第6期512-514,共3页
Acta Universitatis Medicinalis Anhui
基金
安徽省十五科技重点专项(编号:01803016)
安徽省自然科学基金(编号:00144414)
安徽省教育厅高校省学术带头人后备人选科学研究资助项目(编号:2005HBZ18)
关键词
黄芪
中草药/药理学
脑缺血
再灌注损伤
白细胞介素1
白细胞介素6
astragalus membranaceus
drugs, chinese herbal/pharmacology
brain ischemia
reperfusion injary
inter- leukin-1
interleukin-6
tumor necrosis factor
