摘要
目的探讨支气管哮喘大鼠肺组织p38蛋白激酶(p38 MAPK)表达的变化以及黄芪注射液对其影响。方法应用鸡卵清蛋白(OVA)腹腔注射致敏和反复超声雾化吸入刺激复制大鼠哮喘模型。随机分成3组:正常对照组、哮喘模型组和黄芪干预组。分别采用酶联免疫吸附法(ELISA)和蛋白质印迹检测支气管肺泡灌洗液(BALF)IL-5含量和肺组织磷酸化p38 MAPK表达的变化,并观察BALF中EOS计数以及肺组织病理学变化。结果哮喘模型组大鼠肺组织磷酸化p38 MAPK表达水平及BALF中IL-5含量和EOS计数均较正常对照组显著增加(P<0.01);黄芪干预组的上述改变较哮喘模型组显著降低(P<0.01),肺组织病理学损伤程度明显减轻。肺组织磷酸化p38 MAPK表达水平与BALF中IL-5含量和EOS计数之间分别呈显著正相关(r=0.73,0.65,P<0.01)。结论p38 MAPK可能参与了支气管哮喘的发病过程,黄芪对哮喘的治疗作用可能部分与抑制磷酸化p38 MAPK的表达有关。
Objective To explore the effect of astragalus injection on the expression of p38 mitogen-actirated protein kinase （p38 MAPK） in asthmatic rats. Methods Ovalbumin （OVA） was injected intraperitoneally and inhaled to produce the asthmatic model. Twenty-four rats were randomly divided into three groups： control group, asthma group and astragalus group. The concentration of IL-5 in BALF and the expression of phospho-p38 MAPK in lung tissue were measured by ELISA and Western blot, respectively. Results The expression of phospho-p38 MAPK was up-regulated in the lung tissue of asthmatic rats. Compared with asthma group, astragalus injection significantly decreased the expression of phospho-p38 MAPK , the concentration of IL-5 and the number of eosinophil in BALF （ P 〈 0.01 ）, and alleviated the histopathological damage of lung tissue. There were positive correlations between the expression of phospho-p38 MAPK and the concentration of IL-5 and the number of eosinophil in BALF. Conclusion p38 MAPK may play a role in pathological process of asthma. Astragalus could effectively treat asthma by inhibiting the expression of p38 MAPK.
出处
《咸宁学院学报（医学版）》
2009年第1期1-3,共3页
Journal of Xianning Univarsity(medical Sciences)
